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CuCl2 treated groups displayed significantly higher serum Cu levels when compared with the control group and a slight tendency to higher levels was also apparent in the STZ,CuCl2 treated group when compared with the STZ group. In the STZ group on 60th of STZ injection, in accordance with hyperglycemia, GHb 280 270 280 Cu(µg/dL) 260 Cu(µg/dL) 260 240 250 240 220 230 200 220 180 210 1,0 1,1 1,2 1,3 1,4 1,5 1,6 TAOC(mmol/L) 160 180 200 220 240 260 280 Glucose(mg/dL) Fig. 023) Fig. 3 Correlation between plasma copper and TAOC in the ‘‘STZ ?

Arch Toxicol 80: 1–9 Suh SW, Hamby AM, Gum ET, Shin BS, Won SJ, Sheline CT, Chan PH, Swanson RA (2008) Sequential release of nitric oxide, zinc, and superoxide in hypoglycemic neuronal death. J Cereb Blood Flow Metab 28:1697–1706 Takeyama Y, Ogino K, Segawa H, Kobayashi H, Uda T, Houbara T (1995) Effects of zinc on production of active oxygen species by rat neutrophils. Pharmacol Toxicol 76:50–55 Witko-Sarsat V, Rieu P, Descamps-Latscha B, Lesavre P, Halbwachs-Mecarelli L (2000) Neutrophils: molecules, functions and pathophysiological aspects.

This reduces the viscosity of the Histopaque-neutrophils suspension so that the cells can be centrifuged without the need for high g forces). The neutrophils suspension was then centrifuged at 870g for 5 min at 4°C. 25 mL of PBS without Ca2? and Mg2? 25 mL of sterile distilled water was added to the neutrophils pellet to lyse any remaining red blood cells. 2 mL of 3% NaCl. This suspension was then submitted to a new centrifugation at 870g for 5 min at 4°C after which the supernatant was decanted and the neutrophils pellet resuspended in PBS with Ca2?

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Biometals by G. Winkelmann

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