Download e-book for iPad: Cell Culture Methods for In Vitro Toxicology by G. Stacey, Alan Doyle, Margherita Ferro

By G. Stacey, Alan Doyle, Margherita Ferro

ISBN-10: 9048159369

ISBN-13: 9789048159369

ISBN-10: 9401709963

ISBN-13: 9789401709965

Cell tradition equipment for in vitro Toxicology introduces the reader to a number of strategies all in favour of using in vitro telephone tradition in toxicological experiences. It bargains with significant mobile kinds studied within the box of toxicology and may be valuable for an individual wishing to begin paintings with animal telephone cultures or to refresh their wisdom when it comes to in vitro telephone types. basic chapters care for the final biology of cytotoxicity and mobilephone immortalisation those are key concerns for in vitro structures addressing the `3Rs' precept. up to date overviews take care of using cells from liver, mind and gut. moreover, biochemical research of phone responses, biotransformation pathways in cells and recombinant techniques to the early detection of cellphone rigidity also are coated intimately.
famous positive aspects of in vitro applied sciences additionally comprise law, biosafety and standardisation. devoted chapters take care of those matters in a pragmatic manner which will lead the reader to the appropriate resource of knowledge. This e-book presents an up to date, informative and sensible assessment of telephone tradition equipment for in vitro toxicology. it will likely be of equivalent profit to scholars and skilled toxicologists with little adventure of in vitro mobilephone culture.

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G.. Hawley. , Quinto. e.. Collins. M.. Leonard. P. and Goldman, SJ. (1996) Thrombopoietic potential and serial repopulating ability of murine hematopoietic stem cells constitutively expressing interleukin II. Proc. NaIl. Acad. Sci. 93, 10297-10302. Herwig. S. and Strauss, M. (1997) The retinoblastoma protein: a master regulator of cell cycle. differentiation and aqpoptosis, Eur. J Biochem. 246. 581-60 I. e. and Lawlor. PW. (1997) Production of conditionally immortalised cell lines from a transgenic mouse, Audiol.

F. and Bechimol. S. (1999) Alternative pathways for the extention of cellular life span: inactivation ofp53/Rb and expression oftelomerase. Oncogene 18,7676-7680. , LunsdorC H. and Lehmann, J. (1999) Cultivation and characterisation of a new immortalised human hepatocyte cell line, Hep Z, for use in an artificial liver support system, Ann. AT Acad SCI. 875, 364-368. Wu, G. Y. and Wu, CH. (1987) Receptor mediated in vitro gene transformation by a soluble DNA carrier system. J BioI. Chern. 4429-4432.

C o o ~ ..... • -8- ~ ! .. • -10- e' ' ... ••. ' . •.. • -12 • -10 -8 -6 -4 Log 10 IC50 [M1, Microtitration Figure 7. Comparison of IC,os from 6 glioma cell lines exposed to 5 different drugs (bleomycin. VM26. 5-fluorouracil, mithramycin and methyl CCNU) derived by clonogenic assay on a homologous feeder layer or by microtitration autofluorography with methionine as end-point indicator. Reproduced from Freshney's Culture of Animal Cells, a Multimedia Guide, John Wiley & Sons. 1999. by permission of the publishers.

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Cell Culture Methods for In Vitro Toxicology by G. Stacey, Alan Doyle, Margherita Ferro

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